Bacillus firmus is a food safety (GRAS) microorganism determined by the U.S. FDA, and antimbial lipopeptides are antimicrobial substances composed of amino acids. There is no report on their use as feed additives at home and abroad. Through years of research it has been found that the addition of antimicrobial lipopeptides in feed can replace the use of antibiotics such as chlortetracycline and oxytracycline, reducing antibiotic residues in animal food. Technically, the use of biotechnology to improve the strain has increased the yield of antimicrobial lipopeptides It can not only use spores as microbial feed preparations but also use antimicrobial lipopeptides to replace antibiotics for feeding. This is a technology that utilizes fermentation in a comprehensive way. ·Acute toxicity test of mice and rats by oral administration, LD50>6000 mg/kg body weight for mice and. ·The results of the mouse sperm deformity and bone marrow cell micronucleus test were negative. ·The results of the rat teratogen test: There was no obvious reproductive toxicity and embryotoxicity in rats. ·The results of the rat 60-day feeding test: There was no subtoxicity in rats. ·The results of the rat chronic toxicity test and carcinogenicity test: There was no chronic toxicity and carcinogenicity in rats